Journal: Pharmaceutics
Article Title: Study of the Early Effects of Chitosan Nanoparticles with Glutathione in Rats with Osteoarthrosis
doi: 10.3390/pharmaceutics15082172
Figure Lengend Snippet: Micrograph of the knees immunostained for collagen 2A1 (10×) and metalloproteinase-13 (20×) for the different treatments: Hartmann solution ( A – D ), acetic acid ( E – H ), Np-Q ( I – L ), Np-GSH ( M – P ) and hyaluronic acid ( Q – T ).
Article Snippet: Tissue samples were evaluated for indirect immunostaining with the mouse monoclonal antibodies Col2A1 (M2139) and MMP-13 (C-3) from Santa Cruz Biotechnology, using a biotinylated anti-mouse secondary antibody (Donkey anti-mouse IgG (H + L) Biotin, Millipore, Burlington, MA, USA) and the avidin–biotin complex (ABC Elite PK-6100, Vector, Burlingame, CA, USA).
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